CUSTOM BAC LIBRARIES
Our custom large-insert genomic DNA BAC Libraries have average insert sizes between 115 Kb and +180 Kb. We are dedicated to maximum quality and have made over 1,400 custom BAC libraries and processed +50 million high quality BAC clones since 1996.Read more or Request a quote
WHOLE GENOME PROFILING (WGP)
Physical mapping using cleverly-pooled BAC clones and Illumina NGS sequencing. We deliver DNA sequence-based BAC contigs/scaffolds: a chromosome-specific physical map for the whole genome! WGP is a great way to make super contigs for new or ongoing Whole Genome Sequencing projects. WGP is fingerprinting of the future!Read more or Request a quote or Published WGP Manuscript
Amplicon Express has made BAC Libraries
from organisms including:
WHY BAC CLONES ARE STILL NEEDED:
-- BAC clones are from one specific allele (~100 to 200 Kb stretches).
-- There are no Single Nucleotide Polymorphisms (SNPs) on BACs.
-- Sequencing BAC clones can simplify a complex genome assembly to ~100 to 200 Kb fragments.
Allele-specific assemblies are critical for an accurate genome. Bioinformatic leaders strongly support using allele-specific technologies for all genome assemblies--human, personalized medicine, plants and animals--into the foreseeable future. NGS on BAC clones enables allele-specific sequencing and accurate assemblies.
If you're assembling NGS reads onto a reference genome (a "haploid mosaic of different DNA sequences from each donor") and chromosome specificity is unimportant, BACs are probably not necessary.
We can help you determine a cost-effective research strategy, based on your goals, even if you don't need BACs. In most cases, however, a correct genome assembly should be from each allele!