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Read more or Request a quote or Published WGP Manuscript
Amplicon Express has made BAC Libraries
from organisms including:
WHY BAC CLONES ARE STILL NEEDED:
-- BAC clones are from one specific allele (~100 to 200 Kb stretches).
-- There are no Single Nucleotide Polymorphisms (SNPs) on BACs.
-- Sequencing BAC clones can simplify a complex genome assembly to ~100 to 200 Kb fragments.
Allele-specific assemblies are critical for an accurate genome. Bioinformatic leaders strongly support using allele-specific technologies for all genome assemblies--human, personalized medicine, plants and animals--into the foreseeable future. NGS on BAC clones enables allele-specific sequencing and accurate assemblies.
If you're assembling NGS reads onto a reference genome (a "haploid mosaic of different DNA sequences from each donor") and chromosome specificity is unimportant, BACs are probably not necessary.
We can help you determine a cost-effective research strategy, based on your goals, even if you don't need BACs. In most cases, however, a correct genome assembly should be from each allele!