Prepared DNA for Fosmid Library construction: 15-35ug of DNA with 50% of the DNA above 40kb in length. DNA for Illumina library construction: 1ug per library construction. DNA for PacBio 10 to 15ug per library.
Plant Tissue Collect the recommended amount of desired tissue. Wrap the tissue with aluminum foil. It is helpful to separate the tissue into 2 or 3 smaller packages. Label the package(s) with the following information: Organism name Date collected … Continued
All materials, regardless of organism, should be freshly-collected, immediately snap frozen in liquid nitrogen (or a dry ice ethanol bath), and stored at -80C until shipped on dry ice. For specific organisms, see details below. Plants: We have the best … Continued