What is genomic DNAvct library screening?

Genomic DNA library screening is identifying a region of interest on a BAC or Fosmid clone from the target genome. Amplicon Express specializes in library screening. You simply provide any known information–we design and optimize probes, build screening tools (Nylon Filters or Pools & Superpools) and screen the resource for you. We can use pre-existing genomic DNA libraries, or create custom libraries.

What kind of screening does Amplicon Express perform, and how much library coverage is needed?

This will depend on the nature of the known DNA sequence data and the number of screenings needed. When you tell us about your project, we will help you determine the appropriate library, coverage and screening tools. Depending on the project, we may recommend:

  • Pools & Superpools (PCR-based screening)
  • Nylon filters (screening by DNA/DNA hybridization)
  • Custom library screening services

We can also make calculations of the probability of finding a particular locus on a clone with libraries with 1X, 3X, 5X, or +10X coverage, to help you determine the amount of genomic coverage needed.

What do I need to submit for a project with Amplicon Express?

If you already have a genomic DNA library, you simply need to submit ~1Kb to ~2Kb of high-quality genomic DNA sequence data for each region of interest. Amplicon will design appropriate primers/probes for identifying the region of interest on a genomic DNA clone.

Contact us to get a gDNA Screening Services and Tools quote.