Tgenic Event Identification in Plant Breeding Stacks

FOUR STEPS USING BAC CLONES TO CONFIRM TGENIC EVENTS:

Step 1 Make a Custom BAC Library from the Plant Breeding Stack leaf tissue and pick/pool a strategic number of BAC clones.

Step 2 AEX optimizes probes and builds screening tools at the same time your Breeding Stack BAC library is being made.

Step 3 Using DNA/DNA hybridization, AEX scientists identify long gDNA BAC clones containing your Event(s) of Interest.

Step 4 AEX confirms the location of BAC clone ends & sequences the Event directly to verify Tgenic locus presence & position.

Click here to get a quote for your Plant Breeding Stack Tgenic Events.

Or Download a PDF with technical details.

ADVANTAGES OF UTILIZING AMPLICON EXPRESS:

• Some Tgene targets can be difficult or impossible to amplify by PCR (AEX strategy does not rely on PCR for Event confirmation).

• BAC clones can simplify highly repetitive complex genomes down to only ~100Kb to 200Kb fragments.

• BAC clones allow you to determine haplotype & phase of each chromosome/allele.

• AEX lab is compatible with GLP requirements for regulatory approval.

• AEX has optimized Genome Reduction protocols to minimize # of clones screened.

• AEX can also use Fosmid cloning systems when needed (due to project constraints or HMW gDNA availability).

• Multiple Events can be screened simultaneously in a fast and cost effective manner.

• BAC clones serve as a permanent repository of your Tgenic Events (ideal for government agency approval).

• Amplicon Express (AEX) is world renowned for its excellence in BAC library construction and have made over 2,600 BAC libraries since 1996!

• AEX protocols are optimized for constructing high quality BAC libraries with fast turnaround times.

• This means your Breeding Stacks can be screened & Tgenic Events sequence-confirmed within a few weeks!

Details about Genome Reduction Strategy (to minimize cost):

Contact us to get a quote for your Plant Breeding Stack Tgenic Events.