All materials, regardless of organism, should be freshly-collected, immediately snap frozen in liquid nitrogen (or a dry ice ethanol bath), and stored at -80C until shipped on dry ice. For specific organisms, see details below. Plants: We have the best … Continued
All materials, regardless of organism, should be freshly-collected, immediately snap frozen in liquid nitrogen (or a dry ice ethanol bath), and stored at -80C until shipped on dry ice. For specific organisms, see details below. Plants: We have the best … Continued
All materials, regardless of organism, should be freshly-collected, immediately snap frozen in liquid nitrogen (or a dry ice ethanol bath), and stored at -80C until shipped on dry ice. For specific organisms, see details below. Plants: We have the best … Continued
Prepared DNA for Fosmid Library construction: 15-35ug of DNA with 50% of the DNA above 40kb in length. DNA for Illumina library construction: 1ug per library construction. DNA for PacBio 10 to 15ug per library.
After collecting and snap freezing your starting materials, you should have one or more 50ml screw-cap centrifuge tube or foil packets in a plastic bag. Place the bag in a small cardboard box and secure the box. Add padding material as … Continued
Plant Tissue Collect the recommended amount of desired tissue. Wrap the tissue with aluminum foil. It is helpful to separate the tissue into 2 or 3 smaller packages. Label the package(s) with the following information: Organism name Date collected … Continued
All materials, regardless of organism, should be freshly-collected, immediately snap frozen in liquid nitrogen (or a dry ice ethanol bath), and stored at -80C until shipped on dry ice. For specific organisms, see details below. Plants: We have the best … Continued
Below you will find some common problems associated with DNA sequencing as well as the possible causes and solutions for these problems. Continued…