Whole Genome Profiling provides a robust framework for physical mapping and sequencing in the highly complex and repetitive wheat genome

Romain Philippe, Frederic Choulet, Etienne Paux, Jan van Oeveren, Jifeng Tang, Alexander HJ Wittenberg, Antoine Janssen, Michiel JT van Eijk, Keith Stormo, Adriana Alberti, Patrick Wincker, Eduard Akhunov, Edwin van der Vossen, Catherine Feuillet View PDF Jan 20, 2012

Genome Research: Sequence-based physical mapping of complex genomes by whole genome profiling

We present whole-genome profiling (WGP), a novel next-generation sequencing-based physical mapping technology for construction of bacterial artificial chromosome (BAC) contigs of complex genomes, using Arabidopsis thaliana as an example. WGP leverages short read sequences derived from restriction fragments of two-dimensionally pooled BAC clones to generate sequence tags. These sequence tags are assigned to individual BAC […]