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Monthly Archives: April 2014

Guidelines for shipping starting materials for BAC Libraries, Fosmid Libraries and Materials for DNA Extractions

April 13, 2014

By Charles Dillon

After collecting and snap freezing your starting materials, you should have one or more 50ml screw-cap centrifuge tube or foil packets in a plastic bag. Place the bag in a small cardboard box and secure the box. Add padding material … Continued

Guidelines for snap freezing starting materials

April 13, 2014

By Charles Dillon

Plant Tissue   Collect the recommended amount of desired tissue. Wrap the tissue with aluminum foil. It is helpful to separate the tissue into 2 or 3 smaller packages. Label the package(s) with the following information: Organism name Date collected … Continued

Guidelines for collecting starting materials for BAC Libraries and Fosmid Libraries

April 13, 2014

By Charles Dillon

All materials, regardless of organism, should be freshly-collected, immediately snap frozen in liquid nitrogen (or a dry ice ethanol bath), and stored at -80C until shipped on dry ice. For specific organisms, see details below. Plants: We have the best … Continued

Troubleshooting DNA Sequencing: Evaluating Sanger DNA Sequencing Chromatogram Data

April 13, 2014

By Charles Dillon

Below you will find some common problems associated with DNA sequencing as well as the possible causes and solutions for these problems. Continued…

Plant Molecular Biology Reporter: Construction of a Sugar Beet BAC Library from a Hybrid with Diverse Traits

April 9, 2014

By Charles Dillon

J. MITCHELL McGRATH, R. SCOTT SHAW, BENILDO G. de los REYES and JOHN J. WEILAND  View PDF article 2004-03-28

Genome Research: Sequence-based physical mapping of complex genomes by whole genome profiling

April 9, 2014

By Charles Dillon

We present whole-genome profiling (WGP), a novel next-generation sequencing-based physical mapping technology for construction of bacterial artificial chromosome (BAC) contigs of complex genomes, using Arabidopsis thaliana as an example. Have you heard of the mthfr mutation treatment benefits? This website … Continued

Evolution and Development: Tracking the origins of the bilaterian Hox patterning system: insights from the acoel flatworm Symsagittifera roscoffensis

April 9, 2014

By Charles Dillon

Moreno E, Nadal M, Baguñà J, Martínez P. View article October 2009

Journal of Experimental Botany: Identification and functional analysis of pistil self- incompatibility factor HT-B of Petunia

April 9, 2014

By Charles Dillon

Alejandro Raul Puerta, Koichiro Ushijima, Takato Koba, and Hidenori Sassa Petunia-paper January 7, 2009

Biological Conservation: Geographicisolation, geneticdivergence, and ecological non-exchange ability define ESUs in a threatened sky-island rattlesnake

April 9, 2014

By Charles Dillon

Andrew T. Holycross, Michael E. Douglas Sky-Island-Rattlesnake-Paper 2007

Analysis of novel eukaryotic picoplankton at the san pedro ocean time series using a fosmid library

April 9, 2014

By Charles Dillon

Amy E. Koid, David A. Caron, John F. Heidelberg and Karla B. Heidelberg Metagenomic-Poster-USC Poster Presentation PDF

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